Protocols

    Current/prospective users are invited to contact the Optical Imaging Core Facility (OICF) staff at (773) 834-8465 or the Technical Director at (773) 702-7854, to discuss their specific imaging needs and other technical support questions. Prior to scheduling their first imaging session, users must also download and complete an OICF Imaging Request / PI ACUP Amendment Form (see below). Users should then fax their completed OICF IVIS-200 Imaging Request Form to the attention of Dr. Souris at (773) 834-7316, along with signed/approved copies of their pertinent IACUC/IBC protocols and amendments. For imaging requests involving new or amended IACUC/IBC protocols, the OICF IVIS-200 Imaging Request Form first needs to be approved by the OICF’s Internal Advisory Committee (IAC) and the Animal Resources Center (ARC). OICF staff will confirm the receipt and subsequent approval/denial of all imaging requests by e-mail.

    Once a user has been notified by email of his/her OICF IVIS-200 Imaging Request Form’s approval by the OICF/ARC, the user can begin to schedule imaging sessions on-line at http://oicf.uchicago.edu/phpscheduleit2/web. Users can continue to schedule subsequent imaging sessions on-line without completing additional OICF IVIS-200 Imaging Request Forms only if nothing on the original form (e.g., animal strain/transport/biohazard-status), or IACUC/IBC protocol(s)/amendment(s), has changed.

    As noted in the “Services” webpage, imaging can then be conducted either with the technical support of the OICF’s Animal Imaging Specialist during normal business hours, or after-hours (without OICF technical support) by investigators (or their staff) who have been trained by the OICF’s Animal Imaging Specialist and certified by the OICF’s Technical Director.

    Forms

    OICF Imaging Request / PI ACUP Amendment Form

    Policies

    • An approved OICF Imaging Request / PI ACUP Amendment Form completed and submitted by each User to the OICF, must be on file with the OICF before scheduling imaging.
    • A copy of each User's approved IACUC/IBC protocols, and any subsequent amendments to those protocols, must be on file with the OICF before scheduling imaging.
    • Infectious/pathogen-bearing samples (active or inactive, in vivo or in vitro) cannot be imaged at this time, due to instrument decontamination issues and constraints of standing facility protocols. “Infectious/pathogen-bearing” is defined as any agent that is potentially infectious/pathogenic to either humans or animals (as per IBC/IACUC statutes). Infrastructure modifications to accommodate pathogen-bearing specimens are currently under development.
    • Radioactive materials and hazardous chemicals, of any kind, are strictly prohibited from entering the facility.
    • To maintain the facility’s smooth operation and meet multiple/diverse User demands, all imaging during regular business hours (8:30 AM - 5:00 PM) will be conducted by the Animal Imaging Specialist on-call (with the User present, if so desired). After-hours unsupervised use of the OICF will be permitted only to certified users; individuals who have undergone OICF training and testing. Certified User privileges are limited, non-transferable, and will be revoked if facility protocols are not observed.
    • Each Certified User will be held liable for all direct and indirect costs associated with instrument repair/decontamination that arises from improper or unauthorized instrument/facility use.
    • All Users must schedule their imaging studies via the OICF website (here). Each User can reserve no more than 2 sessions (each up to 4 hours length) in advance. Additional instrument time will be granted only if no other User sessions are scheduled for the desired time-slot.
    • Cancellation of reserved imaging sessions must be made at least 4 hours in advance, or the User will be charged for the entire session as scheduled.
    • Scheduled sessions for which the User is more than 30 minutes late will be cancelled and assigned to others (or billed to the original User, if such re-assignment cannot be made).
    • The File Server (Dell 9100C) is only for image analysis and the temporary storage of experimental results. At the end of each imaging session, the Animal Imaging Specialist or Certified User must transfer all that session’s data from the IVIS 200 WorkStation (Dell Xenon) to the File Server. Once transferred to the File Server, the original IVIS 200 WorkStation files will then be deleted. Files on the File Server will be maintained by the OICF for 30 days following their date of creation, after which time they will be deleted without owner notification. Backup copies of File Server files will be available from the Office of Shared Research Facilities IT Group (x4-3191) for up to 90 days following their date of creation, after which time they too will be deleted without owner notification. Subsequent retrieval of files stored off-site, by Iron Mountain, will be available for a fee for 3 years following their date of creation.
    • Facility personnel have the right to reserve time for instrument/facility servicing and may, if necessary, cancel scheduled user sessions without warning. All such scheduling changes will be posted on the OICF scheduling webpage at http://flowcity.bsd.uchicago.edu/php/optical/Scheduler/index.php, which Users are encouraged to review immediately before their scheduled imaging session.
    • Users are responsible for the transfer of their samples/animals to and from the OICF in accordance with BSD, ARC, IBC, and IACUC protocols.
    • All Users must comply with all applicable OSHA regulations and must report any observed facility problems (e.g., hardware, software, safety) to the Technical Director and ARC as rapidly as possible.
    • A current list of available services and associated fees can be found on the OICF website (http://oicf.uchicago.edu/services.php).

    Adobe Reader is required to download all forms and protocols. Download Adobe Reader at http://adobe.com/.